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Questions about ploidy estimate

Open ysbioinfo opened this issue 1 year ago • 0 comments

Hi FACETS developers, When I use FACETS on my WES data, I realized that the ploidy estimate (fit$ploidy) can be much different from the CN segment profile. Below I attached an example:

image

As you can see, most large segments lie on CN2 or CN3 horizontal lines, but the ploidy given by FACETS is 5.14 (purity = 0.38). I just want to make sure this is not a bug. Maybe ploidy is inferred independent of absolute copy number, am I right?

If that is true, which one should I use as the "mean ploidy"?

  1. The value from fit$ploidy
  2. The mean value of absolute CNs of all segments, weighted by the segment length

Do you have any recommendation? Thanks in advance!

Best Yang

ysbioinfo avatar Feb 28 '23 06:02 ysbioinfo