AssertionError: Can only do stranded operations when both PyRanges contain strand info

[leli-li]

I use LAPA on aligned pacbio data from minimap2

I got the following error:

File "/usr/nzx-cluster/apps/lapa/python3.8.11/bin/lapa", line 8, in sys.exit(cli_lapa()) File "/usr/nzx-cluster/apps/lapa/python3.8.11/lib/python3.8/site-packages/click/core.py", line 1157, in call return self.main(*args, **kwargs) File "/usr/nzx-cluster/apps/lapa/python3.8.11/lib/python3.8/site-packages/click/core.py", line 1078, in main rv = self.invoke(ctx) File "/usr/nzx-cluster/apps/lapa/python3.8.11/lib/python3.8/site-packages/click/core.py", line 1434, in invoke return ctx.invoke(self.callback, **ctx.params) File "/usr/nzx-cluster/apps/lapa/python3.8.11/lib/python3.8/site-packages/click/core.py", line 783, in invoke return __callback(*args, **kwargs) File "/usr/nzx-cluster/apps/lapa/python3.8.11/lib/python3.8/site-packages/lapa/main.py", line 112, in cli_lapa lapa(alignment, fasta, annotation, chrom_sizes, output_dir, File "/usr/nzx-cluster/apps/lapa/python3.8.11/lib/python3.8/site-packages/lapa/lapa.py", line 497, in lapa _lapa(alignment) File "/usr/nzx-cluster/apps/lapa/python3.8.11/lib/python3.8/site-packages/lapa/lapa.py", line 297, in call df_cluster = self.annotate_cluster(df_cluster) File "/usr/nzx-cluster/apps/lapa/python3.8.11/lib/python3.8/site-packages/lapa/lapa.py", line 155, in annotate_cluster df = self.create_genomic_regions().annotate(gr) File "/usr/nzx-cluster/apps/lapa/python3.8.11/lib/python3.8/site-packages/lapa/genomic_regions.py", line 66, in annotate gr_ann = pr.PyRanges(gr.df, int64=True).join( File "/usr/nzx-cluster/apps/lapa/python3.8.11/lib/python3.8/site-packages/pyranges/pyranges_main.py", line 2433, in join dfs = pyrange_apply(_write_both, self, other, **kwargs) File "/usr/nzx-cluster/apps/lapa/python3.8.11/lib/python3.8/site-packages/pyranges/multithreaded.py", line 207, in pyrange_apply assert ( AssertionError: Can only do stranded operations when both PyRanges contain strand info