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SaveH5Seurat failing on example mouse bone marrow dataset
Hey! This issue is linked with the scVelo tutorial which uses seurat-disk.
I used the exact code form the tutorial and came across this error:
> SaveH5Seurat(bm, filename = "mouseBM.h5Seurat")
Creating h5Seurat file for version 3.1.5.9900
Error in attr$write(robj) : HDF5-API Errors:
error #000: H5A.c in H5Awrite(): line 676: buf parameter can't be NULL
class: HDF5
major: Invalid arguments to routine
minor: Bad value
Thanks in advance for any assistance!
sessionInfo()
R version 4.0.3 (2020-10-10)
Platform: x86_64-apple-darwin17.0 (64-bit)
Running under: macOS Catalina 10.15.7
Matrix products: default
BLAS: /System/Library/Frameworks/Accelerate.framework/Versions/A/Frameworks/vecLib.framework/Versions/A/libBLAS.dylib
LAPACK: /Library/Frameworks/R.framework/Versions/4.0/Resources/lib/libRlapack.dylib
locale:
[1] en_GB.UTF-8/en_GB.UTF-8/en_GB.UTF-8/C/en_GB.UTF-8/en_GB.UTF-8
attached base packages:
[1] stats graphics grDevices utils datasets methods base
other attached packages:
[1] SeuratWrappers_0.3.0 SeuratDisk_0.0.0.9015 Seurat_3.2.3
loaded via a namespace (and not attached):
[1] Rtsne_0.15 colorspace_1.4-1 deldir_0.1-29 ellipsis_0.3.1 ggridges_0.5.2
[6] rstudioapi_0.11 spatstat.data_1.4-3 leiden_0.3.3 listenv_0.8.0 remotes_2.2.0
[11] ggrepel_0.8.2 bit64_4.0.5 RSpectra_0.16-0 fansi_0.4.1 codetools_0.2-16
[16] splines_4.0.3 polyclip_1.10-0 jsonlite_1.7.1 ica_1.0-2 cluster_2.1.0
[21] png_0.1-7 uwot_0.1.10 shiny_1.5.0 sctransform_0.3.1 BiocManager_1.30.10
[26] compiler_4.0.3 httr_1.4.2 assertthat_0.2.1 SeuratObject_4.0.0 Matrix_1.2-18
[31] fastmap_1.0.1 lazyeval_0.2.2 cli_2.1.0 later_1.1.0.1 htmltools_0.5.1.1
[36] prettyunits_1.1.1 tools_4.0.3 rsvd_1.0.3 igraph_1.2.6 gtable_0.3.0
[41] glue_1.4.2 RANN_2.6.1 reshape2_1.4.4 dplyr_1.0.2 rappdirs_0.3.1
[46] tinytex_0.26 Rcpp_1.0.6 spatstat_1.64-1 Biobase_2.50.0 scattermore_0.7
[51] cellranger_1.1.0 vctrs_0.3.4 nlme_3.1-149 lmtest_0.9-38 xfun_0.18
[56] stringr_1.4.0 globals_0.13.1 ps_1.4.0 mime_0.9 miniUI_0.1.1.1
[61] lifecycle_0.2.0 irlba_2.3.3 goftest_1.2-2 future_1.19.1 MASS_7.3-53
[66] zoo_1.8-8 scales_1.1.1 pcaMethods_1.80.0 promises_1.1.1 spatstat.utils_1.17-0
[71] parallel_4.0.3 RColorBrewer_1.1-2 reticulate_1.18 pbapply_1.4-3 gridExtra_2.3
[76] ggplot2_3.3.2 rpart_4.1-15 stringi_1.5.3 velocyto.R_0.6 BiocGenerics_0.36.0
[81] pkgbuild_1.1.0 rlang_0.4.10 pkgconfig_2.0.3 matrixStats_0.57.0 lattice_0.20-41
[86] ROCR_1.0-11 purrr_0.3.4 tensor_1.5 patchwork_1.0.1 htmlwidgets_1.5.2
[91] cowplot_1.1.0 bit_4.0.4 processx_3.4.4 tidyselect_1.1.0 RcppAnnoy_0.0.18
[96] plyr_1.8.6 magrittr_2.0.1 R6_2.5.0 generics_0.1.0 DBI_1.1.0
[101] pillar_1.4.6 withr_2.3.0 mgcv_1.8-33 fitdistrplus_1.1-1 survival_3.2-7
[106] abind_1.4-5 tibble_3.0.4 future.apply_1.6.0 crayon_1.3.4 hdf5r_1.3.3
[111] KernSmooth_2.23-17 plotly_4.9.2.1 grid_4.0.3 readxl_1.3.1 data.table_1.13.2
[116] callr_3.5.1 digest_0.6.27 xtable_1.8-4 tidyr_1.1.2 httpuv_1.5.4
[121] munsell_0.5.0 viridisLite_0.3.0
Same error.
I have the same error as well. Any help would be appreciated!
Same error.