mixcr
mixcr copied to clipboard
How to align/assemble the targert sequence of IHG ,which dose not contain the V region ?
Hi, I use multiplex PCR to get the target region of IHG.The target region only contains D and J region . When I use the command as follows: mixcr align --report ${output_align_report} ${fasta_rawdata} ${output_align_vdjca} mixcr assemble --write-alignments -f --report ${output_assemble_report} ${output_align_vdjca} ${output_assemble_clna} mixcr assembleContigs --report ${output_assembleContig_report} ${output_assemble_clna} ${output_assembleContig_clns} I get the result which contain V region clonetypes. I want to get the result which only contains D and J region clonetypes(which does not V region ). I look forward to hearing from you .