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How to modify header in order to use M4 option?
Hi mikessh:
Thanks for sharing this great tool. I am trying to use mageri to call variants with the libraries containing UMIs. I have three fastq files generated from bcl2fastq. Two reads for pair ends, and one read for UMI. This is how fastq file containing UMIs looks like: @SN638:1063:HTVFJBCXX:1:1115:18632:43696 2:N:0:ATGCCTAA GAGTTGCGCT + <<AG.GGIG< This is the fastq for one of the pair end read: @SN638:1063:HTVFJBCXX:1:1115:18632:43696 3:N:0:ATGCCTAA GTGGAGTCACAGCGGAGATAGTGCCCTGGCCCTGGGCTTGTGGGGCTGCCCAGCAGCTGCCCATAAAGGACCTGATCGCTGGTGCCAGACTGGGATTGG + <GGGIIIIIIIIGIIIIIIIGIGGIIIIIIGIIIIIIIIIIIIIIGIIIIGIIIIIIGGGGGGGIIGGGIIIIGIIIIIIIIIIGGIIIIIIIIGAGGG
The read from pair end fastq does not contain UMI. Based on Mageri's manual, I am trying to use M4 option, but not sure how to modify the header. Any suggestion?
Thanks for your help, Best Rosemarie