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UniFrac
Currently the UniFrac distance calculation is ported from phyloseq to work with TreeSummarizedExperiment objects. I recently came across this issue in phyloseq github https://github.com/joey711/phyloseq/issues/956
It seems that there are some differences in calculation between qiime2 and phyloseq. Have anyone tested this? Is it possible to check if it is also the case with tse?