Cannot find FragGeneScan

[bheimbu]

Hello,

I've installed MetaCoAG 1.1.4 with mamba but it cannot find run_FragGeneScan.pl, while it is present. I've not experienced something like this before.

$ mamba activate mags
(mags)$ which run_FragGeneScan.pl 
/home/uni08/bheimbu/miniforge3/envs/mags/bin/run_FragGeneScan.pl

I've no idea what's going here.

2024-04-09 11:54:50,795 - INFO - Welcome to MetaCoAG: Binning Metagenomic Contigs via Composition, Coverage and Assembly Graphs.
2024-04-09 11:54:50,796 - INFO - Input arguments: 
2024-04-09 11:54:50,796 - INFO - Assembler used: megahit
2024-04-09 11:54:50,796 - INFO - Contigs file: results_paired_end/assemblies/preprocessed_contigs/BEC327/BEC327.fna
2024-04-09 11:54:50,796 - INFO - Assembly graph file: results_paired_end/MAGs/metacoag/BEC327/BEC327.gfa
2024-04-09 11:54:50,796 - INFO - Contig paths file: None
2024-04-09 11:54:50,796 - INFO - Abundance file: results_paired_end/MAGs/metacoag/BEC327/BEC327.abundance.tsv
2024-04-09 11:54:50,796 - INFO - Final binning output file: results_paired_end/MAGs/metacoag/BEC327
2024-04-09 11:54:50,797 - INFO - Marker gene file hmm: auxiliary/marker.hmm
2024-04-09 11:54:50,797 - INFO - Minimum length of contigs to consider: 1000
2024-04-09 11:54:50,797 - INFO - Depth to consider for label propagation: 10
2024-04-09 11:54:50,797 - INFO - p_intra: 0.1
2024-04-09 11:54:50,797 - INFO - p_inter: 0.01
2024-04-09 11:54:50,797 - INFO - Do not use --cut_tc: False
2024-04-09 11:54:50,797 - INFO - mg_threshold: 0.5
2024-04-09 11:54:50,797 - INFO - bin_mg_threshold: 0.33333
2024-04-09 11:54:50,797 - INFO - min_bin_size: 200000 base pairs
2024-04-09 11:54:50,797 - INFO - d_limit: 20
2024-04-09 11:54:50,798 - INFO - Number of threads: 20
2024-04-09 11:54:50,798 - INFO - MetaCoAG started
2024-04-09 11:54:50,901 - INFO - Total number of contigs available: 2013
2024-04-09 11:54:50,903 - INFO - Total number of edges in the assembly graph: 33
2024-04-09 11:54:50,910 - INFO - Total isolated contigs in the assembly graph: 1952
2024-04-09 11:54:50,910 - INFO - Obtaining lengths and coverage values of contigs
2024-04-09 11:54:50,961 - INFO - Total long contigs: 2013
2024-04-09 11:54:50,961 - INFO - Total isolated long contigs in the assembly graph: 1952
2024-04-09 11:54:50,961 - INFO - Obtaining tetranucleotide frequencies of contigs
2024-04-09 11:54:51,008 - INFO - Scanning for single-copy marker genes
2024-04-09 11:54:51,008 - ERROR - FragGeneScan does not exist. Please install from https://omics.informatics.indiana.edu/FragGeneScan/

Cheers Bastian