bwa errors during local installtion

[ranelmalka100]

Hello :)

I'm trying to locally install crispor, I've started by installing all the required packages according to the requirments.txt file, and it ended successfully :)

Next, I tried to run crispor.py script, using one of my desired sequences as example, as mentioned here - python ../crispor.py h38 ../ENST00000009589.8.fasta exampleout.tsv. I also followed the instructions in 'Adding a genome'.

I've got these messages and error -

INFO:root: * running on sequence 'ENST00000009589.8', guideLen=20, seqLen=519 INFO:root:Progress G0QukS5zrvfbiD5rGwRh - bwasw - Searching genome for one 100% identical match to input sequence [E::bwa_idx_load_from_disk] fail to locate the index files ERROR:root:Error: could not run command set -o pipefail; true G0QukS5zrvfbiD5rGwRh noIp&&/aldemaslab/nofar/crisporWebsite/bin/Linux/bwa bwasw -b 100 -q 100 -T 20 /aldemasla b/nofar/crisporWebsite/genomes/h38/h38.fa /var/tmp/primer3Inmd623rvl.txt > /var/tmp/primer3In78bghbla.txt.

I manually downloaded all the index files of the human genome from the proposed link, and tried to run the script, but still got the same error.

Can you please help and explain me what am I doing wrong ><

Thanks in advance, Nofar :-]