Anchoring barcode file for demultiplexing throws an error

[lchen3]

Getting this error:

cutadapt: error: Character 'F' in adapter sequence 'FILE:CHIPDIPOLIGOS.FASTA' is not a valid IUPAC code. Use only characters 'ABCDGHKMNRSTUVWXY'.)

When trying to anchor my barcode file:

cutadapt -l 8 -e 1 -g ^file:ChIPDipOligos.fasta -o bead_{name}.1.fastq.gz -p bead_{name}.2.fastq.gz bead_split_R1.fastq.gz bead_bc.fastq.gz

When I remove the anchor it runs fine.

Thanks!!

[marcelm]

You’re using an older Cutadapt version - please update!