UserWarning: Warning: representative contig wasn't circularized

[Qikiiso]

Hello, I am getting the following error when running the tool on a plant sample. I don't see any prior reference to this and was wondering if you have any advice on how to proceed.

2024-04-04 02:39:10 [INFO] 10. Building annotation plots for all contigs /disk1/xsq/software/MitoHiFi/src/getReprContig.py:197: UserWarning: Warning: representative contig wasn't circularized repr_contig_id, repr_contig_cluster = get_repr_contig_info(cdhit_out_clstr, rel_mito_len, rel_mito_num_genes, debug=debug) Traceback (most recent call last): File "/disk1/xsq/software/MitoHiFi/src/mitohifi.py", line 565, in main() File "/disk1/xsq/software/MitoHiFi/src/mitohifi.py", line 490, in main plot_annotation.plot_annotation("final_mitogenome.gb", "final_mitogenome.annotation.png") File "/disk1/xsq/software/MitoHiFi/src/plot_annotation.py", line 39, in plot_annotation ax, _ = graphic_record.plot(figure_width=10, strand_in_label_threshold=7) File "/home/xsq/miniconda3/envs/mitohifi_env/lib/python3.7/site-packages/dna_features_viewer/GraphicRecord/MatplotlibPlottableMixin.py", line 438, in plot fig, ax = plt.subplots(1, figsize=(figure_width, height)) File "/home/xsq/miniconda3/envs/mitohifi_env/lib/python3.7/site-packages/matplotlib/pyplot.py", line 1177, in subplots fig = figure(**fig_kw) File "/home/xsq/miniconda3/envs/mitohifi_env/lib/python3.7/site-packages/matplotlib/pyplot.py", line 546, in figure **kwargs) File "/home/xsq/miniconda3/envs/mitohifi_env/lib/python3.7/site-packages/matplotlib/backend_bases.py", line 3358, in new_figure_manager return cls.new_figure_manager_given_figure(num, fig) File "/home/xsq/miniconda3/envs/mitohifi_env/lib/python3.7/site-packages/matplotlib/backends/_backend_tk.py", line 888, in new_figure_manager_given_figure window = tk.Tk(className="matplotlib") File "/home/xsq/miniconda3/envs/mitohifi_env/lib/python3.7/tkinter/init.py", line 2023, in init self.tk = _tkinter.create(screenName, baseName, className, interactive, wantobjects, useTk, sync, use) _tkinter.TclError: couldn't connect to display "localhost:10.0"

I'm running the following command:

nohup python /disk1/xsq/software/MitoHiFi/src/mitohifi.py -r YTJ.ccs.fastq.gz -f B.variegata-mitochondrion.fasta -g sequence.gb -t 36 -a plant -o 1

Can you help me resolve this issue?

Good luck!