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Questions related to interpretation results phased assembly.

Open rfinkers opened this issue 3 months ago • 9 comments

I'm attempting an assembly (relatively heterozygous diploid genome; 5Gb haploid size; with Hifi, ONT & Hi-C).

In the directory 7-consensus, the uniting-popped fast = 7.3Gb; however the uniting.popped.haplotype1, haplotype2 and unsigned is 0 bytes in size.

Thus this imply that there is no contribution of the ONT data to the phasing; though there are reads in the ont_subset.fasta.gz?

The final assembly.fasta is still 7.3Gb; with the haplotype1 and 2 fast both being 1.3G in size. This phasing is the sole contribution from the Hi-C data?

What would be good parameters to re-atempt this assembly; to deal with the higher diversity in this species (compared to human)?

Tnx!

rfinkers avatar Apr 05 '24 08:04 rfinkers