Krona chart using diamond blast

[spatroca]

Hello all,

I am try make Krona chart using blast form diamond. I used the command to DAA output, however, I didn't got convert this file to running in the Krona. Anybody can help me?

Thanks!

[WardDeb]

Hi, We use krona for the exact same purpose. You can convert the DAA to regular blast output using the diamond view option. This converted output can be used directly with krona.

[spatroca]

Thanks, I got convert DAA file using diamond view option. However, I use: ktImportTaxonomy file.input -o file.out.html to plot Krona chart, but showed chart Krona with 100% no hits. I need set any other parameter in this command?

[ashishdamania]

Not sure if this will work: tryktImportBLAST

Also, I wonder if you used --salltitles option when running Diamond.

[spatroca]

I used diamond blastx -d viral.protein.dmnd -q contig.fa -o file -f 100 --salltitles -k 1 after used diamond view -a file.daa -o file_2 returned several row like contig-100_1345 ref|NP_955119.1| 98.4 62 1 0 18 203 1 62 2.4e-28 124.0 I try use ktImportBLAST, but doesn’t work.

[ashishdamania]

Did you try running updateTaxonomy.sh

Is it installed with all helper files? https://github.com/marbl/Krona/wiki/Installing

[spatroca]

Yes, and create taxonomy.tab.

And when I try use ktImportTaxonomy I found in the path.