malton-ont
malton-ont
Hi all, Thank you all for reporting this. It does appear that a regression has slipped in to dorado 0.8.0 where the identified regions for adapter/primer trimming and for barcode...
@rowi2024, Yes, those commands look correct. * Yes, the output from the basecaller should be the input to the demux command * Barcode trimming removes everything outboard of the barcode....
This issue should be resolved in dorado 0.8.1, which has just been released.
@ChristianKniep, We don't maintain the `spack` package, you'd need to contact the maintainers.
Reoccurence of https://github.com/nanoporetech/dorado/issues/400
We haven't been able to conclusively identify the root cause of this issue, though it appears to be related to system settings for Windows 11. You could try using `git...
Raised again in https://github.com/nanoporetech/dorado/issues/1162 - what is being requested is the intended behaviour, so I've changed this from `enchancement` to `bug`.
Thanks for your patience. This issue is resolved in dorado 0.9.0.
@KunFang93 , Those commands looks correct for r10.4.1 data. Note that your output above appears to indicate that your data is r9.4.1 - basecalling with the r10.4.1 model is not...
This is presumably a variant on https://github.com/nanoporetech/dorado/issues/1020. Also note: you are performing barcoding twice. You only need to specify `--kit-name` to _either_ `dorado basecaller` _or_ to `dorado demux` - your...