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MACS -- Model-based Analysis of ChIP-Seq

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**Describe the bug** Python 3.10 has a change, I think in cython where use_tracing is now replaced by tracing. It causes failure to install at Prob.c, MACS2/IO/Parser.c and probably more....

Bug Report

Hi, I have access to bigWig file from an ATACseq experiment, but I do not have the original fastqs nor the bam files of the alignment. I wanted to know...

General Question

**Describe the bug** The code checks to see if the Python version is less than 3.6. It does this by using the floating point equivalent of the version number. This...

Bug Report

Hi tao, I am using macs call peak, when i set q cutoff with 1 and 0.05, the results show a little different, set "-q 1", with ~1500 peaks with...

### Discussed in https://github.com/macs3-project/MACS/discussions/522 Originally posted by **luised94** September 19, 2022 Wasnt sure if it should be on issue or discussion board. Hello, I have been trying to use MACS3...

**Describe the solution you'd like** I'd like the option to calculate scores for peaks when running MACS3 HMMRATAC, similar to the original HMMRATAC implementation (the old command line option `--score...

Feature Request

Hello, I am using macs3 hmmratac mode for ATAC-Seq samples without control samples. I am getting 0 value for columns 13 through 15 in accessible peaks output. Is this related...

General Question

Hi, I have the same question as in (https://github.com/macs3-project/MACS/issues/592#issue-1867137129) 1. and in general, are the columns generated here the same as in the original paper? (https://github.com/LiuLabUB/HMMRATAC/blob/master/HMMRATAC_Guide.md#commandline-options), 2. Another question: In...

General Question

**Use case** I run macs2 with below command per q-value cut-off 0.05, 0.1 and 0.5 `macs2 callpeak -f BAMPE -g hs -n sample_ID -t sample_remove_duplicate_mito.bam --outdir ./peak --min-length 100 --q...

General Question

Hello, I'm working with a single cell ATAC dataset in an organism with a very large genome (32 Gbp). Therefore, the number of reads is quite high ~300M and I...

Feature Request