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degCovariates ERROR
Hi! I am following the workflow to use degCovariates() with a DESeqDataSet object and, when I write:
degCovariates(counts = counts(dds), metadata = as.data.frame(colData(dds)),
fdr = 0.1, scale = FALSE, minPC = 5,
correlation = "kendall", addCovDen = TRUE, legacy = TRUE,
smart = TRUE, method = "lm", plot = TRUE)
I obtained this error:
running pca and calculating correlations for:
un-scaled data in pca;
pve >= 5%;
kendall cor
Error in is.data.frame(x) :
(list) object cannot be coerced to type 'double'
Any ideas what could have gone wrong? Thank you very much in advance!
Hi,
sorry about that. Can you save your objects in an R file and send it to me? I can take a look at what is happening.
cheers!