TomoNet

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Note: This package is actively been updated. I recommend user using Git to install this package such that you can use Git pull to easily catch up the updates.

Installation and Mannual

Please check this user manual for usage of TomoNet: https://mercurial-carriage-b95.notion.site/TomoNet-Tutorial-Version-08302023-66c15fe1c9d24d50a03696700890fe10?pvs=4

Tutorial Data and some related files

https://drive.google.com/drive/folders/11-C55NptELHsv0vi-ZCuFckbLx4un8a1?usp=sharing

Contact

Any issues related to usage of TomoNet can be posted here or sent to email: [email protected]

Google Group

Google group: https://groups.google.com/g/tomonet_cryoet

Reference

This software is supported by NIH grant, and the Paper is published here: http://dx.doi.org/10.1017/S2633903X24000060. Please cite our work if it helps with your data processing, thanks!

Please also cite the corresponding tools/packages if specific modules were used in your workflow:

Motion Correction: MotionCorr2

3D Reconstruction: Imod, PEET, or AreTomo

CTF Est.: CTFFIND4

Manual Picking: Imod

Auto Exp.: PEET

Missing wedge compensation.: IsoNet

Examples of TomoNet Application

HIV Gag 2D lattice

Gag Hexamer STA using 4 tomograms

Microtubule Doulets 1D lattice

S-layer 2D lattice

TomoNet Applications in Other Publications

LCMV GPC

Rabies Virus M Protein

S-layer lattice of M. hungatei