lmanchon
lmanchon
--Hi Mahmoud, i have a dataset i can send you to test the calculation, this dataset was used by Vandesompele et al. to develop their algorithm and to determine the...
--Hi, you can dowload the file directly from this link: https://static-content.springer.com/esm/art%3A10.1186%2Fgb-2002-3-7-research0034/MediaObjects/13059_2001_453_MOESM1_ESM.txt related paper: https://doi.org/10.1186/gb-2002-3-7-research0034 Laurent --
geNorm is well documented here: https://genorm.cmgg.be/
--Hi, right. Maybe you can download raw Cq from this page: https://doi.org/10.1371/journal.pone.0122515 under table S1.
--Yes, you can do it, try with this tool: https://github.com/zqfang/QPCR good luck