database

[zhichusun]

hi!

do you know what the issue is?

Traceback (most recent call last): File "train_plasforest.py", line 174, in main(sys.argv[1:]) File "train_plasforest.py", line 103, in main blast_launcher(inputfile, blast_table, verbose, nthreads, database) File "train_plasforest.py", line 126, in blast_launcher stdout, stderr = blastn_cline() File "/root/.local/lib/python3.6/site-packages/Bio/Application/init.py", line 574, in call raise ApplicationError(return_code, str(self), stdout_str, stderr_str) Bio.Application.ApplicationError: Non-zero return code 2 from 'blastn -out /root/PlasForest/test.fasta_blast.out -outfmt 6 -query /root/PlasForest/test.fasta -db plasmid_refseq.fasta -evalue 0.001 -num_threads 1', message 'BLAST Database error: No alias or index file found for nucleotide database [plasmid_refseq.fasta] in search path [/root/PlasForest::]'