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Software for painlessly estimating average nucleotide diversity within and between populations

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Hi, I used: bcftools mpileup -f reference.fa -b bamlist.txt -r chr10 | bcftools call -m -Oz -f GQ -o chr10-bcf.vcf.gz to get all sites vcf Then I used command: pixy...

help wanted

**Describe the bug** A clear and concise description of what the bug is. **A reproducible example of the bug** Please include the following so we can debug the issue: (1)...

bug

Hello, thank you for developing such a great software. I noticed two issues during my use of the manual: 1. GATK4 also generates multiple alleles, which can interfere with calculations...

help wanted

I have a fairly mundane need for pi and fst estimates that are in the ballpark but not necessarily the most accurate possible. We have a huge number of samples...

help wanted

Hello, thanks for making such a useful tool! I am trying to run pixy to calculate pi on a masked vcf file that will often have one haplotype masked (i.e....

bug

Thank you for this tool! The idea is pretty elegant and we have been needing the "official" script to do these simple stats for so long... I understand that this...

enhancement
todo
high priority

**Is your feature request related to a problem? Please describe.** The problem is that pixy seems unable to handle sex chromosomes. For example, the X chromosome is homogametic and thus...

enhancement
todo
high priority

Hello, it's a great honor to communicate with you. In the process of using Pixy, it is very difficult for me to find the data in the sample script you...

enhancement
todo

Hi! First, thank you for the nice program! I am currently working with lentil WGS data and the vcf cannot be tbi indexed but I found that Pixy does not...

enhancement
todo

Dear Kieran, Thank you for this wonderful tool! I have been using Pixy with standard, well-annotated VCFs, and everything goes correctly. Nevertheless, when I process my VCFs with PLINK, the...

help wanted