Memory Problems with ONT Data

[eprdz]

I was using isONclust in parallel as a previous step to define a transcriptome using ONT data with isONform. I looked at the memory profiling of isONclust and after a few minutes, when almost reaching the memory limit (125 Gb), the memory consumption of isONclust dropped at 40-50 Gb. IsONclust "seems to be working" as the command line did not appear and no error was thrown but only 1 thread out of the total that were running was actually alive. I realized that there were 2 reads that were very long (<100 Kb), while the other reads were 10 Kb long at most. I removed those outlayers and now it seems to work.

I was thinking that maybe some error might be thrown in order not to lead to confusion.

Thanks for your time!