BESST_RNA
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Attribute error?
Hi,
I mapped my RNA reads to my assembly with Hisat2 and fed it into BESST_RNA. However, I got the following error.
Starting scaffolding with library: DARW_heart.hisat2.sorted.bam Parsing BAM file... Computing parameters not set by user...
Mean of library set to: No mean calc since RNA reads Standard deviation of library set to: No std calc since RNA reads -T (library insert size threshold) set to: 20000 -k set to (Scaffolding with contigs larger than): 500 Number of links required to create an edge: 5 Read length set to: 81.4333193897 Relative weight of dominating link set to (default=3): 3
L50: 28205 N50: 30511 Initial contig assembly length: 3235877917
Nr of contigs/scaffolds included in scaffolding: 519724
Total time elapsed: 5.56891298294
Traceback (most recent call last):
File "/home/munhua/installers/BESST_RNA-master/src/Main.py", line 247, in
Any ideas or solutions would be much appreciated.
Thanks Mun