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Published 20 hours ago • verified publisher icon ksahlin

usage questions

Open mictadlo opened this issue 5 years ago • 4 comments

HI, I am working on a hybrid assembly for a 3.2G allotetraploid plant genome:

  1. Illumina assembly with w2rap-contigger.
  2. DBG2OLC to combine Illumina assembly with PacBio reads
  3. PBjelly gap closing with PacBio reads.

Q1. After which above steps do you recommend to run your tool? Q2. Do you recommend to prepare the RNAseq data e.g. with afterqc and any khmer recipes before running your tool? Q3. We have few datasets of different RNA-seq experiments do you think I should combine all or run your tool each time with a different RNA-seq experiments?

Thank you in advance,

Michal

mictadlo avatar Sep 17 '18 23:09 mictadlo