How to find DEG within a cluster between different condition by a Seurat object without 'ind' column?

[Zhr-Abdi]

Hi,

Thanks for your informative scripts and videos. I have a question about the Pseudobulk method. My Seurat obj doesn't have any information about the replicates. It means that each cell has just information about the cluster and the condition (e.g. ctrl or treat) belonged to but not the individual information (for example belonging to which biological replicate). In this situation how can I find DEG within a cluster between two conditions? What is your suggestion?

I would really appreciate it if you could help me.