Tuning the parameters (perc-zero, perc-undetected, pearson) for a relatively small dataset

[ZianFang]

Dear Chromosight developers: First I'd like to thank you guys for developing such an excellent tool to call loop, and it is undoubtedly one of the best and popular loop callers among the users！ It is really necessary to call valid and precise loop to perform downstream analysis, so I am trying to apply chromosight to my Micro-C datasets, which is about 150M contacts for the mouse genome. I know it's a bit of an awkward size because it is a bit smaller than the lowest recommended size, but I still want to have a try. I have read about the closed issues and get to know that I may need to adjust the parameters (perc-zero, perc-undetected, pearson). But what can I do to assess the quality of loops called under different parameters? (I can only come up with this --- visualize the map and see with my eyes ). Can you give me some instructions on fine-tuning and assessing the parameters and the outcome? Or could you please share some professional experience? Best wishes! Woody