Samtools-htslib-API: bam_index_build2() not yet implemented and no index available for pileup

[Dongdong-Chen]

when i run the ./bs3-call_methylation using test data, there have a question. please tell me why? thanks Samtools-htslib-API: bam_index_build2() not yet implemented Traceback (most recent call last): File "/TJNAS01/CANCER/RD/TEST/chendongdong/bs3/bs3-call_methylation.py", line 163, in for col in sorted_input.pileup(): File "pysam/calignmentfile.pyx", line 1087, in pysam.calignmentfile.AlignmentFile.pileup (pysam/calignmentfile.c:12475) ValueError: no index available for pileup