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Error in Sam file
The application output sam format only, the option for bam file exist but wasn't implemented. -f bam doesn't work.
The sam file has an error too, Any buddy understand this line.... Look like the same read map to two chromosome and written to the same line....
NS500599:122:HYY5WBGX2:3:12410:21586:16399 0 4 83813929 70 93M * 0 0 TTTATGAGGGAGGTATTNS500599:121:HYVLGBGX2:4:13606:12721:18500 0 11 126043545 70 112M * 0 0 TGTTAAGGAGTTTTAATTTTATTTTTAGGTGATATGTTGTTATTGGAGGATTTATTTGGGAAGTGTTTAGGTTATATGTGTATTTTAGAAAGATTTTTTTGATTTTATTGGA EEEEEE/EEEEEEEEEEEEEEEEEEAEEAAAEEEEEEEEE/EEEEEEEAE/EEEEEEAEEEEEEEEEEEE<AAEEAEEEAEEEAEE<AEEEEEEEEEEEEEEE<EEAEEA<A PG:Z:SNAP NM:i:0 RG:Z:FASTQ PL:Z:Illumina PU:Z:pu LB:Z:lb SM:Z:sm