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AverageHeatmap() function in Seurat v5
Hello, thanks for developing this wonderful visualization package! I have encountered some issues passing the Seurat v5 object to AverageHeatmap()
function. Could you please advise how should I pull data from the data
layer instead of from slot? Thank you!
> AverageHeatmap(object = pbmc,
+ markerGene = markers$gene)
As of Seurat v5, we recommend using AggregateExpression to perform pseudo-bulk analysis.
This message is displayed once per session.
Warning: No layers found matching search pattern provided
[1] "Your cluster annotation color is:" "#B969E5FF" "#BD94EDFF"
[4] "#E6C0FBFF" "#E477F5FF" "#FFC8CEFF"
[7] "#B8FD8AFF" "#7CF8E1FF"
Warning message:
The `slot` argument of `AverageExpression()` is deprecated as of Seurat 5.0.0.
ℹ Please use the `layer` argument instead.
ℹ The deprecated feature was likely used in the Seurat package.
Please report the issue at <https://github.com/satijalab/seurat/issues>.
This warning is displayed once every 8 hours.
Call `lifecycle::last_lifecycle_warnings()` to see where this warning was generated.