Localization PSF width distribution has gaps

[loekloek]

• Picasso version: 0.6.9
• Python version: Windows install/3.10.13
• Operating System: Windows

Description

After performing localization, I see strange 'holes' in my sx and sy distribution.

What I Did

I use Picasso localize to localize and fit my nd2 movie. I have a z-calibration file based on astigmatism and use the parameters I attached below. I noticed strange z distributions and investigated further. First I thought the problem might be with my calibration, however, I noticed that sx and sy distributions (the sizes of the PSF in x and y of the localizations) show that certain PSF shapes seem to not exist. You can find the histograms for the z distributions and the sx and sy distributions attached below, which I made using Picasso Filter after the localizations.

I tested it both on the Windows install and on the PyPi install and both gave the same result. Also, I see similar holes in datasets from different measurements on different days. Could there be something in the code that would 'filter' out certain PSF shapes?

Let me know if you need any extra info! Thanks for your help in advance :).

Parameters: configtxt.txt Sx, sy: Z: