ncov-tools
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align2alleles.py will skip fixed variants when --min-allele-count is greater than 1
When align2alleles.py is called with --min-allele-count > 1 and a SNP is present in all samples, the reference allele will not be included here:
https://github.com/jts/ncov-tools/blob/master/workflow/scripts/align2alleles.py#L82
This is not unintuitive so should be changed so the reference allele is always included