nanocorrect
nanocorrect copied to clipboard
Experimental pipeline for correcting nanopore reads
Hi! When i run nanocorrect-overlap.make i get this error: make -f nanocorrect-overlap.make INPUT=~/MinION/sspace_butyricum/all_reads_buty.fasta NAME=cor nanocorrect-preprocess.pl /home/mattia/MinION/sspace_butyricum/all_reads_buty.fasta > cor.pp.fasta fasta2DB cor cor.pp.fasta File cor.pp.fasta, Line 1502272: Warning: Read is longer than...
"No # -sign in source name" error occurred. ``` $(NAME).las: HPCcommands.txt /bin/bash $^ LAcat $(NAME) > $@ rm $(NAME).*.las ``` I think the input method has changed in LAcat. I...
Hello, trying the tool for the first time. @OHRI-bell-virt ~/programs/nanocorrect $ make -f nanocorrect-overlap.make INPUT=/scratch/apelin/MinION_sequencing/Kevin_plasmid/MinION/Pass_2D_seqtk.fasta NAME=nc nanocorrect-preprocess.pl /scratch/apelin/MinION_sequencing/Kevin_plasmid/MinION/Pass_2D_seqtk.fasta > nc.pp.fasta fasta2DB nc nc.pp.fasta DBsplit -s50 nc DBdust nc HPCdaligner -t5...
The daligner allow the error rate to be as high as 0.3, but still much lower than nanopore rate. So the sensitivity is not high enough. For example in the...