#error regarding cellchat object

[surinderchd]

Dear Sir/Madam,

i am having this error while working with cellchat, please guide mr to solve this error,

cellchatnew <- createCellChat(object = PTstroma_traj, group.by = "Group", assay = "RNA") [1] "Create a CellChat object from a Seurat object" The meta.data slot in the Seurat object is used as cell meta information The cell barcodes in 'meta' is AAACGCTCATGCCGAC-1_1 AACCAACCAACTAGAA-1_1 AACCTGACAAATCCCA-1_1 AACGTCATCTTTGCAT-1_1 AACTTCTTCCCGAGGT-1_1 AACTTCTTCGCGAAGA-1_1 Error in .rowNamesDF<-(x, value = value) : invalid 'row.names' length In addition: Warning messages: 1: In LayerData.Assay5(object = x, layer = i) : multiple layers are identified by data.BDP3_db/m.1 data.1 data.BDP4_db/db.2 data.2 data.BDP5_SGLT2i.3 data.3 data.BDP6_SGLT2i-aPC.4 data.4 data.BDP7_db/m.5 data.5 data.BDP8_db/db.6 data.6 data.BDP9_SGLT2i.7 data.7 data.BDP10_SGLT2i-aPC.8 data.8 only the first layer is used 2: In createCellChat(object = PTstroma_traj, group.by = "Group", assay = "RNA") : The cell barcodes in 'meta' is different from those in the used data matrix. We now simply assign the colnames in the data matrix to the rownames of 'mata'!

[sqjin]

@surinderchd It looks like you are working on a Seurat v5 object. It should work if you re-install the package.

[surinderchd]

Dear Sir @sqjin , Thank you for your reply, do i need to reinstall seurat or cellchat?