How CellChat v2 compute autocrine interactions on spatial transriptomics data

[ynpan910]

Hello,

Thanks for the amazing tool which I have been using on scRNA-seq and now on spatial transcriptomics data. I have one question about the CellChat v2 which expands to incorporate spatial locations into the inference.

I read the preprint, especially the Figure 4 which illustrates how CellChat v2 incorporate spatial locations to identifies cell-cell communications between two cell groups. I wonder how the autocine cell-cell communications are calculated? Is it the same, which perhaps by considering spatial locations of the cells within one cell group? Figure 4d says the spatial plot only shows paracine signaling interactions, but maybe this is not related to the math behind but just for visualization purpose.

Thanks for your time, and I would appreciate any education!