estimated library size of NucleosomeFree.bam is unexpectedly small

[cparsania]

Hi,

After shift and splitting bams according to fragment size, I see amongst all bam the largest bam is NulceosomeFree.bam which is expected. However, when I run estLibSize (from ChIPpeakAnno package), the output is <1000 which is smaller than the output of mononucleosome.bam which is in millions although the size of bam is smaller than NuclesomeFree.bam. can you tell me what's wrong here. ?? Attached here is the plot showing library size