Data without pseudotime

[DHelix]

Hi, This may be a naive question - Is the pseudotime information absolutely required for IReNA analysis? I'm interested in inferring cell-type-specific regulatory networks from a heterogeneous population (e.g. tumor microenvironment). I do not expect one cell type will progress to another cell type, so it doesn't make sense to me to reconstruct the trajectory. Would you please give me some advice? Thanks!