About Spatial plot of single gene :

[10KGenomics]

Dear sir

I'm running data_ plotter.py script, the instructions are as follows:

data_ plotter. py --counts ./Sample_ Tr.tsv --xlim 50 --ylim 50 --show-genes Penk

Because the script defaults to the X axis limits to have equally sized sub images (default: [1, 33]) and the Y axis limits to have equally sized sub images (default: [1, 35]).

My spot size is 50 μ M (50 / 50 = 2500 spots), the error is as follows:

How do I optimize code?