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extract_kraken_reads.py No reads processed
I've just downloaded the code and run extract_kraken_reads.py this way:
./KrakenTools-master/extract_kraken_reads.py -k output.kraken -s R1.fastq -s2 R2.fastq -o R1_extracted.fastq -o2 R2_extracted.fastq -t 2 --exclude --report report.txt --fastq-output
but I got this error:
PROGRAM START TIME: 11-19-2021 08:54:26
1 taxonomy IDs to parse
>> STEP 1: PARSING KRAKEN FILE FOR READIDS AZTI526.kraken
0.00 million reads processed
0 read IDs saved
>> STEP 2: READING SEQUENCE FILES AND WRITING READS
0 read IDs found (0.00 mill reads processed)
0 read IDs found (0.00 mill reads processed)
0 reads printed to file
Generated file: AZTI526_R1_extracted_kraken.fastq
Generated file: AZTI526_R2_extracted_kraken.fastq
Why no read was processed?
I got same issue , no read processed :
any suggestion?
Same here. Have you solved the problem?
Try adding -r kraken.report --include-children
.
I've included both of those flags, and I'm still having the problem.
You sure to be using the right input? For example the file [sample].kraken instead of [sample].kraken.report?