canu polishing with CLR reads

[botellaflotante]

Hello, I have a canu assembly with CLR reads (ca. 30X coverage). I used racon for polishing with the same reads... I got as a result much larger contigs. I don't have a reference genome to compare if what racon did I right as it seems based on contig lengths... Is this approach supposed to work well, or only illumina reads are recommended for improving this kind of assembly?

thanks

[rvaser]

Hello, it can happen that Racon extends a given assembly. Try to evaluate with BUSCO or if you have cdna data run paftools asmgene.

Best regards, Robert