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error: overlap is not transmuted!

Open Valentin-Bio-zz opened this issue 3 years ago • 2 comments

Hello I installed racon via conda conda install racon

I made my SAM file using bwa.

then I run racon: racon -t 20 joined_reads.fastq UlvaAlignContIlu.sam ulvacomp.contigs.fasta > polished_contigs.fasta

I got this error message:

error: overlap is not transmuted!

why im getting this? I took a look into this: https://github.com/isovic/racon/issues/77 but after 3-4 years idk if this still being a solution

THanks for your time :)

Valentin-Bio-zz avatar Dec 29 '21 17:12 Valentin-Bio-zz

Hello, can you please verify which version you are using with racon --version? Also, please paste commands which produced the .sam file.

Best regards, Robert

rvaser avatar Dec 30 '21 15:12 rvaser

I had this issue come up with 4 out of >200 assemblies that I've run through Racon. I'm using v1.4.20. The one thing that I'm noticing is that they all contain a number of smaller contigs with abnormal coverage. I removed contigs smaller than 50kb from the assembly which seems to do the trick. It'd be nice though to be able to pass these through without getting the cryptic error.

gettl008 avatar Jan 31 '22 16:01 gettl008