Unequal lengths in sequence and overlap file

[marco91sol]

Hi,

I have installed racon 1.4.20 version through conda.

Firstly, I used minimap2 for mapping my corrected PacBio reads vs genome assembly. Then I ran racon with the following parameters:

racon -m 8 -x -6 -g -8 -w 500 -t 64 pacbio_reads.fasta mapping.sam de_novo_assembly.fa > racon.fasta.

It gives me the following mistake:

How can I solve it?

Thanks for your help!

Br, Marco

[rvaser]

Hi Marco, how did you run minimap2?

Best regards, Robert