different clustering results

[YuliaInn]

Hello,

I integrated some data using Harmony back in 2022 (on Seurat V4), but I didn’t save the Seurat object at that time. When I needed to perform further analysis, I reran Harmony on the same data on Seurat V5, but the clustering results were quite different. Specifically, the number of clusters decreased from 20 to 18, and most importantly, I can’t find cluster 17, which is crucial for our analysis.

Is there any way to recover the clustering results from 2022? If reproducing the previous results isn't possible, I plan to increase the resolution parameter until I see the cluster of interest and annotate the new clusters. How can I justify that the new annotation closely matches the previous one?

Can it be because of Seurat version?

Thank you for your help!