Error message: Can't find files for block a expected files such as

[Amanda-Biocortex]

Hi,

Im getting a DADA2 error message when running lotus2 (DADA2 + lambda) on some paired reads. Ive pasted some of the log output below. One thing I noticed was that pair2 is being trimmed at a very high precent value (the in the example below its 6.49042e+06% end - trimmed.

At ERR36_1.fastq:

Writing demultiplexed files to : ./lotus2/err/output//tmpFiles//demultiplexed/ 50.5841% of 66175 reads accepted (99.0812% end-trimmed) Pair 2: 6.49042e+06% of 66174 reads accepted (6.49042e+06% end - trimmed) ...

Reads processed: 5,374,129; 5,374,306 (pair 1;pair 2) Rejected: 3,177,307; 5,374,763 Accepted (High qual): 2,197,369; 0 (64,093; 5,374,738 end-trimmed) Accepted (Mid qual): 340;0 Singletons among these: 0; 0 Bad Reads recovered with dereplication: 2,072,433 Min/Avg/Max stats Pair 1 - sequence Length : 200/200/200 - Quality : 32/36.2312/38 - Median sequence Length : 0, Quality : 0 - Accum. Error 0.202734 Trimmed due to:

25 avg qual_ in 20 bp windows : 0; 0 (0.95) acc. errors, trimmed seqs : 0; 0 Rejected due to: < min Sequence length (200) : 2,847,795; 5,374,741 -after Quality trimming : 2,847,692; 5,374,745 < avg Quality (27) : 0; 0 < window (50 nt) avg. Quality (25) : 27,259; 0 max Sequence length (1000) : 0; 0 (8) homo-nt run : 1,599; 0 (0) amb. Bases : 196; 0 (2.7) binomial est. errors : 327,807; 0 Specific sequence searches: -With fwd Primer remaining (<= 0 mismatches) : 0; 0 -Barcode unidentified (max 0 errors) : 0

SampleID Barcode Instances SMPL0 70,658 SMPL1 51,257 ...

Error: Can't find files for block a expected files such as

Aborting dada2 run Execution halted

Any help on this error message comes up would be greatly appreciated