scRNA-seq

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Hello, I have been following some of the tutorial provided by hbc training specifically on integrating different datasets: https://hbctraining.github.io/scRNA-seq/lessons/06_SC_SCT_and_integration.html I believe I have encountered a slight issue. I followed much...

nasjr08

Thank you so much for your sharing,it has benefited me a lot. I have 10 samples in 2 condition, every conditon has 5 samples. So when to integrate data to...

learnyoung1990

Error in CellsByIdentities(object = object, cells = cells)

1

Hi, I got an error while running the following code ``` # Filter out low quality reads using selected thresholds - these will change with experiment filtered_seurat = 500) &...

luenhchang

https://github.com/hbctraining/scRNA-seq/blob/master/lessons/mitoRatio.md I ran into a problem when I did the last step. metadata$mtUMI

allllany

update the elbow plot

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the elbow plot markdown (quantitave approach) needs a figure update

mistrm82

we should add a markdown? or some text about how we can specify whether to use raw counts or normalized. The following code will allow us to extract the raw...

marypiper

Zhu got the error: 'calculateQCMetrics' is defunct. Use 'perCellQCMetrics' instead. See help("Defunct") She tried perCellQCMetrics but it is not the same as calculateQCMetrics - need to look into this error...

marypiper

finalized decision tree

1

marypiper