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Implement OptiType pre-filtering step

Open tavinathanson opened this issue 8 years ago • 3 comments

After #423 is done, we can consider using a better aligner that doesn't suffer from the following:

You can use any read mapper to do this step, although we suggest you use RazerS3. Its only drawback is that due to way RazerS3 was designed, it loads all reads into memory, which could be a problem on older, low-memory computing nodes.

Suggested by @ihodes in https://github.com/hammerlab/biokepi/issues/419#issuecomment-279821401.

tavinathanson avatar Feb 14 '17 22:02 tavinathanson

Discussion here: https://github.com/hammerlab/biokepi/issues/423

ihodes avatar Feb 14 '17 22:02 ihodes

Per https://github.com/hammerlab/biokepi/issues/419#issuecomment-280073430, it may not be worth it to add this with razerS3 vs. an aligner that won't require 208GB+ of memory (or no possible GCP machine type for high enough depth BAMs).

tavinathanson avatar Feb 15 '17 17:02 tavinathanson

@armish and I agreed that the razers3 pre-filtering step (vs. using a different aligner) doesn't seem particularly high priority, since (a) there's a good chance it wouldn't impact results (and it shouldn't) and (b) it probably wouldn't fix OOM issues.

tavinathanson avatar Feb 16 '17 16:02 tavinathanson