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-M parameter for BWA MEM alignment

Open aariani opened this issue 3 years ago • 0 comments

Thanks a lot for developing this pipeline. I have a question about BWA MEM alignment. In your main paper you mention that:

SpeedSeq aligns paired-end FASTQ files to the human GRCh37 reference genome with BWA-MEM, using the “-M” flag to mark shorter alignments as secondary

However by looking into the code it seems that you just use BWA MEM with the -t -R and -I options

aariani avatar Jan 04 '21 08:01 aariani