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Add flag to check/enforce strandness for a dataset

Open emi80 opened this issue 8 years ago • 1 comments

The pipeline allows to process data with different strandness within the same run. Many time all libraries in a dataset have the same strandness and we would need to (1) check this and stop the pipeline if the automatic strandness inference gives different values or (2) enforce a specific strandness to the process and skip the automatic detection.

The flag could be named --readStrand and have the following values:

  • CHECK for (1)
  • one of NONE, SENSE, ANTISENSE, MATE1_SENSE, MATE2_SENSE for (2)

emi80 avatar Oct 17 '16 10:10 emi80

For the moment, just adding a --read-strand option to enforce a specific strandness to all processed samples.

By default the option is empty ('') and the pipeline will infer strandness as usual. To enforce a configurations one of NONE, SENSE , ANTISENSE, MATE1_SENSE, MATE2_SENSE must be given.

emi80 avatar Dec 13 '17 10:12 emi80