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gtf assembled using stringtie has lines missing strand info

Open linzhangTuesday opened this issue 4 years ago • 2 comments

Hi I used stringtie to assemble a new transcriptome and merge with a reference one.

The output gtf has ~30 lines without strand info in field 7 , compared to ~360k lines are + and - respectively. The downstream software is complaining about "Invalid strand" for those lines missing strand info while parsing the gtf file. Those lines all look like new-assembled with only "MSTRG.#".

Please let me know if there are any suggestions.Thanks in advance!

linzhangTuesday avatar Feb 19 '21 04:02 linzhangTuesday

I have the same issue, in some of the transcripts my strand is simply "." Did you solve this? Thanks!

ghost avatar Mar 02 '21 11:03 ghost