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One cautionary thing about S3: there is rate-limiting on a per-prefix ('folder') level, so you'd need to be careful about how you represented folder structure. One of the attractions of...

[N0.zip](https://github.com/user-attachments/files/17171826/N0.zip) [N2.zip](https://github.com/user-attachments/files/17171932/N2.zip) Of course, and I noted more single gene HOG can be also found in other nodes, for example in my analysis, 1027 single HOG exists in N2.tsv. Thanks...

Hi,Jonathan Many thanks for your kind work. A file recording species name and gene name is also attached, may helps. [SpeciesTree_rooted_node_labels.txt](https://github.com/user-attachments/files/17280252/SpeciesTree_rooted_node_labels.txt) [SpeciesAndGeneName.tsv.gz](https://github.com/user-attachments/files/17280257/SpeciesAndGeneName.tsv.gz) [OG0000056_fasta.txt](https://github.com/user-attachments/files/17280269/OG0000056_fasta.txt)

Hi,Laurie, It is also attached, hope you proceed well. [OG0000056_tree.txt](https://github.com/user-attachments/files/17293685/OG0000056_tree.txt)