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Clinker issue: Blocks instead of arrows

Open clpas opened this issue 2 years ago • 2 comments

When the Genbank input file contain their CDS information within 'join(...)', clinker does not work properly (see picture). Can anyone help me with this problem? Thanks!

Screenshot 2022-10-12 135151

clpas avatar Oct 12 '22 11:10 clpas

We handled this via email, but just for record keeping, this is caused by sequence features spanning the origin in circular sequence records. I implemented some functionality to try and automatically fix these scenarios (https://github.com/gamcil/clinker/commit/16f795f813fb8f29ca4382df0efebcd797be9031) which will be in the next release. However, it will be better to manually adjust the origin/linearise your sequence externally (e.g. in Geneious/SnapGene/UGENE etc) before giving it to clinker.

gamcil avatar Oct 24 '22 02:10 gamcil

this happens to some files after changing the origin for circular phage genomes using Geneious. if you replace the line containing the first instance of the word "join" with source 1..{genome length} and then delete the line containing the second instance of the word "join" that fixes the block issue. however, you lose that feature where the second instance of the word join occurs. p.s. this is one of the most useful and elegant tools I have come across, thank you!!!

pweigele avatar Apr 25 '23 12:04 pweigele