dunovo icon indicating copy to clipboard operation
dunovo copied to clipboard

QC pipeline: Trim ends of reads

Open NickSto opened this issue 5 years ago • 1 comments

Add a trimming step to remove the first and last 5-10bp of each duplex consensus sequence.

Trimmomatic or FASTQ Trimmer are candidate trimmers for this.

NickSto avatar Oct 25 '19 21:10 NickSto

Alternatively, use the MD tag to only filter out variants that occur in that region, not the sequence.

NickSto avatar Oct 25 '19 21:10 NickSto