Volpy GUI unable to load summary images

[cawarwick]

• Tell us a bit about your setup:

1. Windows 10

2. Python version: 3.7.12

3. Working environment: Jupyter Notebook

4. Which of the demo scripts you're using for your analysis (if applicable): demo_pipeline_voltage_imaging.py to get sample data and generate the summary images to test the GUI

5. CaImAn version*: 1.9.6

6. CaImAn installation process: conda

7. Windows machine specs: Intel i7 8700, 32gb RAM, GT 730, nVME drive.

• Describe the issue that you are experiencing I ran the VolPy demo pipeline up to the point at which is saves summary images which are used in the VolPy GUI. This demo downloads demo data, so it the summary images should be generated by the small demo data. I then launched the GUI (volpy_gui.py) and used the GUI to load the file generated by the first part of the dmeo: "demo_voltage_imaging_summary_images.tif". Once I try to load the summary image into the GUI it 'crashes' in that it closes the GUI and the powershell says this: ' WARNING:root:Your tif file is saved a single pagefile. Performance will be affected tree changes:

<ActionParameter 'LOAD DATA' at 0x1dd67eac4c8> activated None '

PART 2: Update on the original issue. I figured I should update and try it again. I updated Caiman using Conda Forge and also updated the caiman_data using the manager and now I get a different issue running the same demo file as above running a Jupyter Notebook. Info below:

TypeError Traceback (most recent call last) ~\AppData\Local\Temp\ipykernel_4796\3448104306.py in 277 # versus from the CLI 278 if name == "main": --> 279 main()

~\AppData\Local\Temp\ipykernel_4796\3448104306.py in main() 154 summary_images = np.stack([img, img, img_corr], axis=0).astype(np.float32) 155 # save summary images which are used in the VolPy GUI --> 156 cm.movie(summary_images).save(fnames[:-5] + '_summary_images.tif') 157 fig, axs = plt.subplots(1, 2) 158 axs[0].imshow(summary_images[0]); axs[1].imshow(summary_images[2])

~\Anaconda3\envs\caiman\lib\site-packages\caiman\base\timeseries.py in save(self, file_name, to32, order, imagej, bigtiff, excitation_lambda, compress, q_max, q_min, var_name_hdf5, sess_desc, identifier, imaging_plane_description, emission_lambda, indicator, location, unit, starting_time, experimenter, lab_name, institution, experiment_description, session_id) 173 if extension in ['.tif', '.tiff', '.btf']: 174 with tifffile.TiffWriter(file_name, bigtiff=bigtiff, imagej=imagej) as tif: --> 175 if "%4d%02d%02d" % tuple(map(int, tifffile.version.split('.'))) >= '20200813': 176 def foo(i): 177 if i % 200 == 0:

TypeError: not all arguments converted during string formatting

[pgunn]

How much RAM do you have?

Also poking: @caichangjia

[caichangjia]

I have talked with him in gitter. The GUI is not tested on windows machine. Will fix this when I have time.

[cawarwick]

32gb of RAM, I will update the post with some basic hardware.

[caichangjia]

So I did find and fix problems in the volpy_gui due to some changes in pyqt. I will ask for a pull request soon. Sorry for the delay. However I didn't manage to reproduce the second problem. The demo works fine on spyder and should work similarly on the jupyter notebook as it is tested in google colab.